Defining replication origin efficiency using DNA fiber assays
نویسندگان
چکیده
منابع مشابه
Analysis of re-replication from deregulated origin licensing by DNA fiber spreading
A major challenge each human cell-division cycle is to ensure that DNA replication origins do not initiate more than once, a phenomenon known as re-replication. Acute deregulation of replication control ultimately causes extensive DNA damage, cell-cycle checkpoint activation and cell death whereas moderate deregulation promotes genome instability and tumorigenesis. In the absence of detectable ...
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The precise position of the replication origin (O(R)) of mtDNA was determined for insect species belonging to four different orders (four species of Drosophila, Bombyx mori, Triborium castaneum, and Locusta migratoria, which belong to Diptera, Lepidoptera, Coleoptera, and Orthoptera, respectively). Since the free 5' ends of the DNA strands of mtDNA are interpreted as the O(R), their positions w...
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Replication of eukaryotic chromosomes initiates at multiple sites called replication origins. Replication origins are best understood in the budding yeast Saccharomyces cerevisiae, where several complementary studies have mapped their locations genome-wide. We have collated these datasets, taking account of the resolution of each study, to generate a single list of distinct origin sites. OriDB ...
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DNA replication is a fundamental biological process that serves to create two copies of the genetic material during each cell division. Complete and precise replication enables identical sets of genes to be faithfully delivered to daughter cells during each cell division. To achieve rapid duplication of the entire genome, eukaryotic cells initiate DNA replication at multiple locations on each c...
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Maintenance of replication fork stability is of utmost importance for dividing cells to preserve viability and prevent disease. The processes involved not only ensure faithful genome duplication in the face of endogenous and exogenous DNA damage but also prevent genomic instability, a recognized causative factor in tumor development. Here, we describe a simple and cost-effective fluorescence mi...
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ژورنال
عنوان ژورنال: Chromosome Research
سال: 2009
ISSN: 0967-3849,1573-6849
DOI: 10.1007/s10577-009-9098-y